Device

Part:BBa_J100224

Designed by: Julia Preziosi   Group: Campbell M Lab   (2015-07-07)


Theophylline Aptamer from PDB with RS3, riboswitch in tCloneRed

This part comes from the combination of a theophylline aptamer, as determined by the Protein Data Bank, with part of "Riboswitch 3" from Wachsmuth (the sequence minus its aptamer) to make a new riboswitch. This riboswitch was inserted into tCloneRed, and functions to express the reporter gene, RFP, when in the presence of its ligand, theophylline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 756
    Illegal AgeI site found at 868
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None